Table 2.

Primers and Probes Used in qRT-PCR

Gene name	Sequence	Strand	Modification
ANKRD22	5′-CCAGCTTGGACTTCTAGGGA-3′	Forward
	5′-GGCAGATGGGCTCAGAGTAT-3′	Reverse
	5′-TCCCATGCTGGTCCTTCACAGG-3′	Probe	5’Fam–3’Tamra
LGR5	5′-AGCAAACCTACGTCTGGACA -3′	Forward
	5′-ATGCTGGAGCTGGTAAAGGT-3′	Reverse
	5′-TCCTGTGACTCAACTCAAGCCTTGGT-3′	Probe	5’Fam–3’Tamra
TUBB	5′-AGGAGGTCGATGAGCAGATG-3′	Forward
	5′-TTGCCAATGAAGGTGACTGC-3′	Reverse
	5′-TGTGACATCCCACCTCGTGGCC-3′	Probe	5’Fam–3’Tamra
Ankrd22	5′-CCAAGGCCTTCATCTCTCCA-3′	Forward
	5′-GGCCAAGTCTTCAGAGGGAT-3′	Reverse
	5′-AGGACCCATTGCTCACGTTGGAATCT-3′	Probe	5’Fam–3’Tamra
Lgr5	5′-TGGGCAAAGTAGGATTCGGT-3′	Forward
	5′- CATCGAACACCTGCGTGAAT-3′	Reverse
	5′-AGCTGTGTCTTGTTTCCGGATCAACCA-3′	Probe	5’Fam–3’Tamra
Mist1	5′-GCAGGACCTACATGTCCCTT-3′	Forward
	5′-TCCTGCTATCCCAGACTCCT-3′	Reverse
	5′-AGGATCTTTAGAGACTCCGGTCCCGT-3′	Probe	5’Fam–3’Tamra
Sox2	5′-TGATGGAGACGGAGCTGAAG-3′	Forward
	5′-TTCTCCTGGGCCATCTTACG-3′	Reverse
	5′-CGCCGTGGCGTTGCCTCCTC-3′	Probe	5’Fam–3’Tamra
Sox9	5′-TTCAGATGCAGTGAGGAGCA-3′	Forward
	5′-CTTGCAGAGGCATGTGTTGT-3′	Reverse
	5′-CCCGTGTCACAACACACGCACA-3′	Probe	5’Fam–3’Tamra
Cd44	5′-GGCACTGGCTCTGATTCTTG-3′	Forward
	5′-CACCGTTGATCACCAGCTTT-3′	Reverse
	5′-CCTACTATTGACCGCGATGCAGACGG-3′	Probe	5’Fam–3’Tamra
Cd133	5′-TGTCAACCTAGAGCAGGCAA-3′	Forward
	5′-TAAACCGCAGGTAGCTCCAA-3′	Reverse
	5′-TGCCCTTCCTTGTGGATTTCTGTCTGC-3′	Probe	5’Fam–3’Tamra
Cd166	5′-AGAAACCGCGTCTACCTTGA-3′	Forward
	5′-CACACCCTTCCTCAGTGTCT-3′	Reverse
	5′-ACTCCCAGGACGGGCGACCC-3′	Probe	5’Fam–3’Tamra
Ssea1	5′-CCTCTTCTGTGACTCGTGGA-3′	Forward
	5′-ATCCCTGCAAAGTAGGCAGT-3′	Reverse
	5′-TGCCCAGTAAACACAAGGCGGCA-3′	Probe	5’Fam–3’Tamra
Ssea4	5′-GGGCGTATAGCAGAACCTCT-3′	Forward
	5′-CCCGTTTCTCATCACAGCAG-3′	Reverse
	5′-CAGGGCTCGAACCATCGCACA-3′	Probe	5’Fam–3’Tamra
Tubb	5′-ACCTTGCCTTACCTCTGACC-3′	Forward
	5′-CACAAGGGCAGGAACATACG-3′	Reverse
	5′-ACCCACTCCTGACCCAGCACC-3′	Probe	5’Fam–3’Tamra

5’Fam, 5(6)-Carboxyfluorescein; 3’Tamra, 5(6)-Carboxytetramethylrhodamine.