TABLE 2.

Bacterial skin count and mortality of various groups examined under different treatment conditions.

Mice groups	#Mean bacterial count (CFU/ml)		Mortality (%)
	1st stage	2nd stage
G1	0.0 ± 0.0a	0.0 ± 0.0a	0.0 ± 0.0a
G2	2.75 × 104 ± 0.5b	1.4 × 104 ± 0.5b	29.6 ± 0.5c
G3	2.58 × 104 ± 0.5b	1.18 × 104 ± 0.5b	9.6 ± 0.5b
G4	5.2 × 103 ± 0.8e	2.6 × 103 ± 0.6c	0.0 ± 0.0a
G5	6.2 × 103 ± 0.4f	4.1 × 103 ± 0.8e	9.6 ± 0.5b
G6	4.9 × 103 ± 0.6e	0.15 × 103 ± 0.4a	0.0 ± 0.0a
G7	3.8 × 103 ± 0.8c	0.0 ± 0.0a	0.0 ± 0.0a
F-value	41.545***	30.271***	2.53***

Independent t-test and values are mean of three replicates ± SD; 1st stage, after 3 days of initial treatment; 2nd stage, at the treatment end time. Means in the same column followed by different letters significantly different at P<0.05, ***P < 0.001.

^#Number of bacteria (CFU/ml) = average number of bacterial colonies/amount plated × dil. Factor.

G1 (negative control) comprised neither burned nor infected mice.

G2 and G3 (positive controls), mice were burnt, and get infected without receiving any treatment (G2) or treated with Placebo gel only (G3).

The mice of G4 were burnt, infected, and treated with Ag-NPs gel, while the mice of G5 were burnt, infected, and treated with neomycin gel. G6 and G7 (neomycin-Ag-NPs gel and spray formulas, respectively) in which mice were burnt, microbial suspensions were applied, and the mice received treatment with neomycin-Ag-NPs gel (G6), and neomycin-Ag-NPs spray (G7), respectively.