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. 2021 Sep 20;8:743013. doi: 10.3389/fmolb.2021.743013

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Copyright © 2021 Han, Li, Jia, Zhao, He, Huang, Jiang, Wu, Yang, Ji, Liu, Chen and Chu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AMO-loaded exosomes were taken up by colon cancer cells through NRP-1. (A) Electron microscope analysis and particle size analysis of exosomes, scale bars = 200 μm. (B) Tissue expression of NRP-1 in a online database (The Human Protein Atlas). A majority of cancer tissues showed weak to moderate cytoplasmic immunoreactivity with a granular pattern, and the expression of NRP-1 was highest in colon cancer. (C) Q-PCR was used to detect the relative expression of NRP-1 in colon cancer cells (HCT116 and Caco2) and 293T cells. (D)The surface expression of NRP-1 in colon cancer cells was detected by flow cytometry. (E) After co-incubation with the FAM-labeled exosomes, the positive rate of cells expressing FAM green fluorescence was detected by flow cytometry. ***p < 0.001. (F) The green fluorescence signal of cells was observed by fluorescence microscopy.