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. 2021 Sep 20;8:743013. doi: 10.3389/fmolb.2021.743013

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Copyright © 2021 Han, Li, Jia, Zhao, He, Huang, Jiang, Wu, Yang, Ji, Liu, Chen and Chu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AMO-loaded exosomes inhibited colon cancer cell proliferation. (A) The relative expression level of miR-221 in colon cancer cells was detected by Q-PCR. Caco2 and HCT116 cells were treated with 20 μg exosomes containing anti-miRNA-221 (AMO-Exos) or their controls (AMO-NCs) for 24 h, and then lysed to detect the expression level of miR-221. (B) Caco2 and HCT116 cells were treated with 20 μg exosome drugs. After continuous culture for different times, the cell proliferation was evaluated. (C) Effect of exosome drugs on clone formation ability of Caco2 cells. Caco2 cells were treated with 20 μg exosome drugs and cultured for 14 days. The number of clones was calculated and histograms were shown. (D)Effect of exosome drugs on HCT116 cell clone formation ability.