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. 2021 Sep 20;12:679135. doi: 10.3389/fmicb.2021.679135

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Copyright © 2021 Yin, Ji, Duan, Tian, Ju, Yan, Zhang and Liu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bacterial pathway biomarkers of lamb age and the rumen microbiota gradually stabilize. (A) The top 21 biomarkers of bacterial pathways were identified by applying RandomForest regression of their relative abundances in lambs against chronological age. Ranking in descending order of importance to the accuracy of the model was to determine biomarker taxa. The insert illustrates the result of the 10-fold cross-validation error. (B) Heatmap of changes over ages showing the relative abundances of the top 21 age-predictive biomarkers for bacterial pathways.