Reagent	Final concentration	Stock concentration	Amount
Tribromoethanol	0.02 g/mL in sterile saline	1.6 g/mL in Tert-amyl alcohol	0.2 mL/10g body weight
Bupivacaine	0.00125 g/mL in sterile saline	0.0084 g/mL in sterile saline	100 μL per mouse
Dexamethasone	0.1 mg/mL in sterile PBS	1 mg/mL in sterile PBS	50 μL/20 g body weight

Store stock solution at 4°C for tribromoethanol for 1 year, and −20°C for bupivacaine and dexamethasone for 6 months.