Figure 5.
Monocyte-derived IL-18 induces PD-L1 expression on tumor-experienced NK cells. (A) Frequency of PD-L1hi NK cells after culture of PBMC in the absence or in the presence of K562, isolated NK cells in the presence of K562 cells and/or autologous monocytes (Mo), in the absence or in the presence of an anti-IL-18 neutralizing mAb for 48 h, assessed by flow cytometry. (B) concentration of IL-18 in supernatants of the cultures, assessed by ELISA. Data represent mean ± SEM, (n=4). One-way ANOVA with Dunnett´s post hoc test. ns, non-significant; *p<0.05, **p<0.01.