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PD-L1hi NK cells exhibit an activated phenotype and express higher cytotoxic mediators. Frequency of CD25+ cells (A), CD69+ cells (B) TRAIL+ cells (C), FasL+ cells (D), CD107a+ cells (E) and IFN-γ + cells (F) detected in PD-L1hi (red) and PD-L1-/low NK cells (blue) after stimulation of PBMC with K562 cells for 48 h, assessed by flow cytometry (n=7). A representative histogram for each marker is shown. Two-sided Student´s t test **p<0.01, ***p<0.001, ****p<0.0001.
