Figure 7.

Tumor-experienced NK cells inhibit CD8⁺ T cell proliferation through PD-L1. (A) Experimental design used to generate control NK cells (NK_control ) and tumor-experienced NK cells enriched in PD-L1^hi cells (NK_te ) to investigate their effect on T cell proliferation. (B) Relative proliferation of CD8⁺ T cells analyzed by CFSE dilution after stimulation with anti-CD3/anti-CD28 mAb and cultured with increasing numbers of autologous NK_control or NK_te . (C) Relative proliferation of CD8⁺ T cells analyzed by CFSE dilution after stimulation with anti-CD3/anti-CD28 mAb in the absence or in the presence of autologous NK_control or NK_te in the absence or in the presence of an anti-PD-L1 blocking mAb. Representative histograms showing CFSE dilution in live CD3⁺CD8⁺CD56^- cells are shown (n=3). Data represent mean ± SEM. Two-way ANOVA with Sidak´s post hoc test (B) and one-way ANOVA and Tukey´s post hoc test (C) *p<0.05, **p<0.01, *** p<0.001.