Fig. 3.

PTEN is a direct target of miR-499a and its expression is upregulated by OGD. (A and B) Both mRNA and protein levels of PTEN were upregulated in OGD astrocytes. **p < 0.01 vs. control; n = 5. (C) The binding site for miR-499a in the 3′-UTR of PTEN gene, as predicted by TargetScan algorithm. The mutant sequences were equivalent to wild-type with the exception of mutation at the 3′ end of the target site. (D) Luciferase activities were analyzed in HEK293 cells 48 h after transfection. The data were presented as mean ± SEM from four separate experiments; *p < 0.05 and *p < 0.05. (E, F) Regulation of mRNA and protein levels of PTEN by miR-499a in astrocytes. **p < 0.01 vs. NC, **p < 0.01 vs. miR-499a; n = 5.