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. 2021 Aug 23;40(19):e107988. doi: 10.15252/embj.2021107988

Figure 3. Transcription initiation at OriL does not depend on the structural elements important for promoter initiation.

Figure 3

  • A, B
    Single‐subunit and multi‐subunit RNA polymerases can transcribe the OriL stem‐loop structure. Transcription assays were run using +1C OriL (template #12) and the human or yeast Saccharomyces cerevisiae mtRNAP and phage T7 RNAP (A), and E. coli core RNAP on WT OriL (B).
  • C
    The mtRNAP structural elements required for promoter initiation are dispensable for the OriL transcription. Transcription assays were performed using LSP or +1C OriL (template #12), as indicated, with the WT mtRNAP or its mutants lacking the intercalating hairpin (ΔIH) or the specificity loop (ΔSP).
  • D
    A structural model of the mtRNAP‐OriL complex. The stem‐loop structure used to represent OriL is shown in pink (13 nt loop region) and blue (8 bp stem region). MtRNAP molecule is depicted as a surface model (yellow) with major subdomains indicated. In this configuration of the complex, the DNA initiating nucleotide is placed in the vicinity of the enzyme’s active center to allow base‐pairing with the priming ATP molecule.