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. 2021 Aug 23;40(19):e107988. doi: 10.15252/embj.2021107988

Figure EV3. PolG and mtRNAP assemble into a ternary complex on WT and −1A OriL.

Figure EV3

  • A, B
    EMSA using OriL and mtRNAP (A) and PolG (B).
  • C
    Relative affinity of mtRNAP and PolG to OriL as observed in A and B.
  • D
    Relative affinity of mtRNAP to WT and −1A OriL assayed using EMSA.
  • E
    The primosome forms on both WT and −1A OriL. EMSA was performed using WT (left) or −1A OriL (right). Note the change of mobility of the labeled species in the presence of both mtRNAP and PolG (“super shift”).
  • F
    Elution profile of PolG‐OriL‐mtRNAP, PolG and mtRNAP during size‐exclusion chromatography on Superdex 200 column.
  • G
    Coomassie stained SDS–PAGE showing the composition of fractions obtained in size‐exclusion experiment with the primosome complex. Molecular weight markers Mark12 (Invitrogen) are show in lane M.