FIGURE 4.

ACK2‐IR cells within 1 μm of a VIP‐IR neuronal fiber were larger than those not within 1 μm of a VIP‐IR fiber, regardless of treatment or challenge with LPS. (a/e) and (b/f) are representative images of ACK2‐immunoreactive cells (red) and VIP‐immunoreactive fibers (green) from a cell <1 μm from a VIP fiber or >1 μm from a fiber, respectively. Quantification in (c) and (d) of the mean ACK2 cell size in μm2 by treatment. (g) and (h) are quantification in the same fashion as (c/d) but in slices challenged with LPS. n = 8 animals Scale bars in (a/e) and (b/f) are all 5 μm