Reagent/Resource	Reference or Source	Identifier or Catalog Number
Experimental Models
Brucella abortus 2308::miniTn7K‐dsRed	Miller et al, 2017	N/A
Brucella abortus 2308 ∆bspF::miniTn7K‐dsRed	This study	N/A
Brucella abortus 2308 ∆bspF::miniTn7K‐dsRed‐bspF	This study	N/A
Brucella abortus 2308 ∆bspB::miniTn7K‐dsRed	Miller et al, 2017	N/A
Brucella abortus 2308 ∆bspB::miniTn7K‐dsRed‐bspB	Miller et al, 2017	N/A
Brucella abortus 2308 ∆virB11::miniTn7K‐dsRed	Smith et al, 2016	N/A
Homo sapiens: embryonic kidney 293T cells (HEK293T/17)	ATCC	Cat#CRL‐11268; RRID:CVCL_1926
Homo sapiens: HeLa cells (CCL‐2)	ATCC	Cat#CCL‐2; RRID:CVCL_0030
Homo sapiens: HeLa‐M clone 1 (C1) cells	Gordon et al, 2010	N/A
M. musculus: Bone Marrow Derived Macrophages (BMMs) from C57BL/6J mice	The Jackson Laboratory	Cat#000664
Recombinant DNA
Indicate species for genes and proteins when appropriate
pUC18T‐miniTn7K‐dsRed‐bspF Brucella abortus	This study	N/A
pUC18T‐miniTn7K‐dsRed	Smith et al, 2016	N/A
pUC18T‐Tn7‐tnp	Dr M Kovach; Myeni et al, 2013	N/A
pcDNA3‐arf6‐HA Homo sapiens	This study	N/A
pCMV‐HA	Clontech	Cat#635690
pCMV‐HA‐bspF Brucella abortus	Myeni et al, 2013	N/A
pCMV‐Myc	Clontech	Cat#631604
pCMV‐myc‐ACAP1 Homo sapiens	This study	N/A
pmCherry‐C1	Clontech	Cat#632524
pmCherry‐N1‐arf6 T27N Homo sapiens	This study	N/A
pmCherry‐bspF Brucella abortus	This study	N/A
pmCherry‐rab6a′T27N Homo sapiens	This study	N/A
pmCherry‐rab8a T22N Homo sapiens	This study	N/A
pEGFP‐ACAP1 Homo sapiens	This study	N/A
pEGFP‐N1‐arf6 Homo sapiens	This study	N/A
pEGFP‐N1‐arf6 Q67L Homo sapiens	This study	N/A
pEGFP‐N1‐arf6 T27N Homo sapiens	This study	N/A
pEGFP‐MICAL‐L1 Homo sapiens	This study	N/A
pEGFP‐rab6a Homo sapiens	This study	N/A
pEGFP‐rab6a′ Homo sapiens	This study	N/A
pEGFP‐rab8a Homo sapiens	Hattula & Peränen, 2000	N/A
pEGFP‐rab11a Homo sapiens	This study	N/A
pEGFP‐stx6 Rattus norvegicus	Kudlyk et al, 2013	N/A
pEGFP‐stx16 Homo sapiens	Willett et al, 2013	N/A
pEGFP‐TGN38	This study	N/A
pEGFP‐VAMP3 Homo sapiens	Addgene	Cat#42310
pEGFP‐VAMP4 Homo sapiens	Addgene	Cat#42313
pCLXSN‐MCS2	Miller et al, 2017	N/A
pCLXSN‐MCS2‐GFP	Miller et al, 2017	N/A
pCLXSN‐MCS2‐GFP‐ACAP1 Homo sapiens	This study	N/A
pCLXSN‐MCS2‐GFP‐ACAP1(R448Q) Homo sapiens	This study	N/A
pCLXSN‐MCS2‐arf6(Q67L)‐GFP Homo sapiens	This study	N/A
pCLXSN‐MCS2‐arf6(T27N)‐GFP Homo sapiens	This study	N/A
pCLXSN‐MCS2‐GFP‐bspF Brucella abortus	This study	N/A
pCLXSN‐MCS2‐mCherry	This study	N/A
pCLXSN‐MCS2‐arf6Q67L‐mCherry Homo sapiens	This study	N/A
pCLXSN‐MCS2‐arf6T27N‐mCherry Homo sapiens	This study	N/A
pCL‐Eco	Imgenex, Novus Biologicals	Cat#NBP2‐29540
Antibodies
Mouse monoclonal anti‐β‐Actin (clone 8H10D10) (1:20,000)	Cell Signaling Technology®	Cat#3700S; Lot#18; RRID:AB 2227609
Rabbit monoclonal anti‐Arf6 (clone D12G6) (1:10,000)	Cell Signaling Technology®	Cat#5740S, Lot#1; RRID:AB_10694539
Rabbit polyclonal anti‐Calnexin (IF 1:1,000) (WB 1:20,000)	Enzo Life Sciences, Inc.	Cat#ADI‐SPA‐860‐D; Lot#12301304; RRID:AB_10616095
Mouse monoclonal anti‐ERGIC‐53 (G1/93) (1:200)	Alexis® Biochemicals, Enzo Life Sciences, Inc.	Cat#ALX‐804‐602‐C100; RRID:AB_2051363
Mouse monoclonal anti‐GM130 (1:500)	BD Transduction Laboratories™, BD Biosciences	Cat#610823; Lot#07536; RRID:AB_398142
Rabbit monoclonal anti‐HA (clone C29F4) (1:10,000)	Cell Signaling Technology®	Cat#3724S, Lot#9; RRID:AB_1549585
Rat monoclonal anti‐HA (3F10) (1:500)	Roche	Cat#1867423; RRID:AB_390918
Mouse monoclonal anti‐Hsp27 (G31) (1:10,000)	Cell Signaling Technology®	Cat#2402; Lot#8; RRID:AB_331761
Rabbit polyclonal anti‐Lamin A/C (1:5,000)	Cell Signaling Technology®	Cat#2032; Lot#5; RRID:AB_2136278
Rat monoclonal anti‐LAMP1 (1D4B) (1:400)	Developmental Studies Hybridoma Bank, University of Iowa	Cat#1d4b; RRID:AB_2134500
Rabbit monoclonal anti‐Myc (clone 71D10) (1:10,000)	Cell Signaling Technology®	Cat#2278S, Lot#5; RRID:AB_490778
Mouse monoclonal anti‐p230 (1:100)	BD Transduction Laboratories, BD Biosciences	Cat#611280; RRID:AB_398808
Rabbit monoclonal anti‐Rab6 (clone D37C7) (1:1,000)	Cell Signaling Technology®	Cat#9625S, Lot#1; RRID:AB_10971791
Rabbit monoclonal anti‐Rab8a (clone D22D8) (1:1,000)	Cell Signaling Technology®	Cat#6975S, Lot#2; RRID:AB_10827742
Rabbit monoclonal anti‐Syntaxin 6 (clone C34B2) (IF 1:100) (WB 1:1,000)	Cell Signaling Technology®	Cat#2869S, Lot#6; RRID:AB_2196500
Peroxidase AffiniPure Goat anti‐Mouse IgG (1:10,000)	Jackson Laboratories	Cat#115‐035‐071
Peroxidase AffiniPure Goat anti‐Rabbit IgG (1:10,000)	Jackson Laboratories	Cat#111‐035‐144
Alexa Fluor™ 488‐conjugated donkey anti‐rabbit (1:500)	Invitrogen, ThermoFisher, Scientific	Cat#A21208, Lot#1810450
Alexa Fluor™ 647‐conjugated donkey anti‐rat (1:500)	Molecular Probes®, ThermoFisher, Scientific	Cat#A21208; Lot#1810450
Alexa Fluor™ 647‐conjugated phalloidin (1:250)	Molecular Probes®, ThermoFisher, Scientific	Cat#A22287; Lot#1246069
Oligonucleotides and other sequence‐based reagents
RC247‐bspF pGBKT7 Forward: 5′‐GATGGAGGCCGAATTCGCTGCAAAACCTTTGCTTGAGG‐3′	This study	N/A
RC248‐bspF pGBKT7 Reverse: 5′‐GCAGGTCGACGGATCCTTTATGCTCGGTGAAACTGCG‐3′	This study	N/A
RC603‐miniTn7K‐dsRed Forward: 5′‐ATCATCCTCATCACCGACAA‐3′	Myeni et al, 2013	N/A
RC604‐miniTn7K‐dsRed Reverse: 5′‐GCTATATTCTGGCGAGCGAT‐3′	Myeni et al, 2013	N/A
WSU0141‐ACAP1 Reverse BamHI: 5′‐TATTGGATCCTATTACAGCGTGTGGAGGTCATG‐3′	This study	N/A
WSU0165‐Mutagenesis ACAP1 R448Q Forward: 5′‐GTTCCGGCATCCACCAGAGCCTTGGTGTTC‐3′	This study	N/A
WSU0166‐Mutagenesis ACAP1 R448Q Reverse: 5′‐GAACACCAAGGCTCTGGTGGATGCCGGAAC‐3′	This study	N/A
WSU0218‐bspF Tn7K Forward: 5′‐AGCTCGAATTCACCATCTTCCGATCTTGGCTG‐3′	This study	N/A
WSU0219‐bspF Tn7K KpnI Reverse: 5′‐AAGGTACCTTATTTATGCTCGGTGAAACTGC‐3′	This study	N/A
WSU0221‐bspF pmCherry Forward BglII: 5′‐CTCAGATCTGCTGCAAAACCTTTGCTTGAGG‐3′	This study	N/A
WSU0222‐bspF pmCherry Reverse KpnI: 5′‐CGCGGTACCTTATTTATGCTCGGTGAAACTGC‐3′	This study	N/A
WSU0247‐eGFP Forward EcoRI: 5′‐TAGCGGAATTCGTCGCCACCATGGTGAGC‐3′	This study	N/A
WSU0248‐rab6a Reverse BamHI: 5′‐CGGGATCCGTTAGCAGGAACAGCCTCC‐3′	This study	N/A
WSU0258‐rab6a T27N Forward: 5′‐CAAAGCGTTGGAAAGAATTCTTTGATCACCAGA‐3′	This study	N/A
WSU0259‐rab6a T27N Reverse: 5′‐TCTGGTGATCAAAGAATTCTTTCCAACGCTTTG‐3′	This study	N/A
WSU0350‐pEGFP‐N1 BamHI Reverse: 5′‐CGCGGATCCGCTTTACTTGTACAGCTCGTCCATG‐3′	This study	N/A
WSU0353‐pEGFP C1 Reverse: 5′‐TGATCAGTTATCTAGATCCGGTGG‐3′	Miller et al, 2017	N/A
WSU0354‐pEGFP C1 Forward ClaI: 5′‐TAATATCGATGCCACCATGGTG‐3′	Miller et al, 2017	N/A
WSU0433‐arf6 Forward EcoRI: 5′‐ATAAGAATTCACCATGGGGAAGGTGCTA‐3′	This study	N/A
WSU0435‐ACAP1 Forward SalI: 5′‐TTAGTCGACATGACGGTCAAGCTGGATTTCG‐3′	This study	N/A
WSU0437‐arf6 Reverse KpnI: 5′‐GAGGTACCAGAGATTTGTAGTTAGAGGTTAACC‐3′	This study	N/A
WSU0438‐rab11a Forward EcoRI: 5′‐ ATTAGAATTCTATGGGTACCCGCGAC‐3′	This study	N/A
RC451‐ACAP1 pCMV Forward EcoRI: 5′‐ATGGAGGCCCGAATTCAAACGGTCAAGCTGGATTTCGAG‐3′	This study	N/A
RC452‐ACAP1 pCMV Reverse EcoRI: 5′‐TCGGTCGACCGAATTCTTACAGCGTGTGGAGGTCATG‐3′	This study	N/A
RC453‐acap1 pGADT7 Forward: 5′‐GGAGGCCAGTGAATTCACGGTCAAGCTGGATTTCGAG‐3′	This study	N/A
RC464‐acap1 pGADT7 Reverse: 5′‐CGAGCTCGATGGATCCCAGCGTGTGGAGGTCATG‐3′	This study	N/A
WSU0620‐MICAL‐L1 Forward HindIII: 5′‐ TAGTAAGCTTATATGGCTGGGCCG‐3′	This study	N/A
WSU621‐MICAL‐L1 Reverse SalI: 5′‐ATTGTCGACTTAGCTCTTGTCTCTGG‐3′	This study	N/A
TW770‐bspF pEGFP‐C1 Forward BglII: 5′‐GTCCGGACTCAGATCTGCTGCAAAACCTTTGCTTGA‐3′	Myeni et al, 2013	N/A
TW771‐bspF pEGFP‐C1 Reverse BglII: 5′‐CTTGAGCTCGAGATCTTTATTTATGCTCGGTGAAACTGCG‐3′	Myeni et al, 2013	N/A
siRNA targeting sequence: ON‐TARGETplus Mouse Arf6 siRNA ‐ SMARTpool: CAAACGGGGUGGGGUAAUA, CUGACAUUUGACACGAAUA, CGGCAUUACUACACCGGGA, GGGUCUCAUCUUCGUGGUA	Dharmacon™	Cat#L‐043217‐01‐0005
siRNA targeting sequences: ON‐TARGETplus Non‐targeting Pool: UGGUUUACAUGUCGACUAA, UGGUUUACAUGUUGUGUGA, UGGUUUACAUGUUUUCUGA, UGGUUUACAUGUUUUCCUA	Dharmacon™	CAT# D‐001810‐10‐20
siRNA targeting sequence: ON‐TARGETplus Mouse Rab6a siRNA ‐ SMARTpool: GGGCGGAGACUUCGGGAAU, UCAGAGGAAGUAUGCAUUA, GCACUUGGAUUAUGGAUCU, UCGUGGAGGUGAUGUAUUA	Dharmacon™	CAT#L‐040858‐01‐0005
siRNA targeting sequences: ON‐TARGETplus Mouse Rab8a siRNA ‐ SMARTpool: CAGGAGCGGUUUCGAACAA, GUAUCAUGCUGGUCUACGA, CAGAAGGUAGCCAGCGGUA, CGGACUCGAUUCACAAAUU	Dharmacon™	CAT#L‐040860‐01‐0005
siRNA targeting sequences: ON‐TARGETplus Mouse Stx6 siRNA ‐ SMARTpool: GCACAUCUAUUACGCUUAU, CAUCACAAGUACUCGGCAA, CUGGAGUGGCAGAUCGCUA, AGAACAUGUCGCAGCGCAU	Dharmacon™	CAT#L‐059391‐01‐0005
Chemicals, Enzymes and other reagents
Cytochalasin D	Thermo Fisher	Cat#C8272‐1MG, Lot#067M4075V
AlexaFluor™488‐conjugated Cholera Toxin Subunit B	Invitrogen	Cat#C22841
Fetal Bovine Serum	Atlanta Biologicals	Cat#S10350H; Lot#J15105
Human epidermal growth factor (EGF)	EMD Millipore	Cat#01‐107
Normal horse serum	Gibco	Cat#16050‐130; Lot#1517706
FuGENE® 6	Promega	Cat#E2692
Rapamycin	LC Laboratories®	Cat#R‐5000; Lot#ASW‐125
Aureobasidin A	Takara Bio USA, Inc	Cat#630499
X‐a‐Gal	Takara Bio USA, Inc	Cat#630463
Software
Include version where applicable
Adobe® Photoshop® CS6 software for Mac	Adobe Systems Incorporated, San Jose, California, USA	www.adobe.com/ products/photoshop; RRID:SCR_014199
CellProfiler4.0.7	Broad Institute, Cambridge, Massachusetts, USA	www.cellprofiler.org
GraphPad Prism® version 9.0e for Mac	GraphPad Software, La Jolla, California, USA	www.graphpad.com; RRID:SCR_002798
Fiji ImageJ 2.1.0	Open source database, National Institute of Health	imageJ.nih.gov
MacVector version 12.7.5 for Mac	MacVector, Inc., Apex, North Carolina, USA	www.macvector.com
Other
Matchmaker Gold Yeast Two‐Hybrid system	Takara Bio USA, Inc	Cat#630489
Mate & Plate human bone marrow cDNA library	Takara Bio USA, Inc	Cat#630477
Yeastmaker™ Yeast Transformation System 2	Takara Bio USA, Inc	Cat#630439
Easy Yeast Plasmid Isolation kit	Takara Bio USA, Inc	Cat#630467
Arf6 G‐LISA Activation Assay Kit (Colorimetric Based)	Cytoskeleton, Inc.	Cat#BK133
Mouse Macrophage Nucleofector® Kit	Lonza	Cat#VPA1009