Fig. 3.

Necroptosis activates a noncanonical ER stress response. (A–D) HT-29 cells were treated with human TNF (50 ng/mL), SM-164 (100 nM), z-VAD.fmk (25 μM), Nec-1s (10 μM), GSK′872 (10 μM), and NSA (2.5 μM) as indicated. The cell lysate was collected and analyzed by immunoblotting using indicated antibodies. The values under the blots indicate quantification of relative density of GRP78 bands.