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. 2021 Sep 20;118(39):e2017460118. doi: 10.1073/pnas.2017460118

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Copyright © 2021 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 6. — Loss of GCS1 leads to loss of self-tolerance. (A and B) Posterior fat body tissue dissected from larvae raised at 25 °C and stained with FITC-WGA to assay protein N-glycosylation. Control c833-GAL4; UAS-GAL4^RNAi tissue is positive for FITC-WGA staining (A). c833-GAL4; UAS-GCS1^RNAi shows a mosaic loss of FITC-WGA staining, where * marks fat body cells with decreased staining (B). (C) tuSz¹; c833-GAL4; UAS-GCS1^RNAi flies raised at 25 °C show melanized self-encapsulations (indicated by the arrow). (D) Penetrance of the self-encapsulation phenotype in control tuSz¹; c833-GAL4; UAS-GAL4^RNAi and tuSz¹; c833-GAL4; UAS-GCS1^RNAi flies raised at 25 °C. *P < 0.05 compared to tuSz¹; c833-GAL4; UAS-GAL4^RNAi. (E) Penetrance of the self-encapsulation phenotype in hop^Tum/+, hop^Tum/+; Mgat1^KG02444/+, and hop^Tum/+; α-Man-IIb^MI09613/+ flies raised at 28 °C. *P < 0.05 compared to hop^Tum/+. (F) hop^Tum/+; Mgat1^KG02444/+ flies raised at 28 °C show melanized self-encapsulations (indicated by the arrow). (G) hop^Tum/+; α-Man-IIb^MI09613/+ flies raised at 28 °C show melanized self-encapsulations (indicated by the arrow). P values (D and E) were determined by generalized linear models.