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. 2021 Sep 8;297(4):101179. doi: 10.1016/j.jbc.2021.101179

Figure 3.

Figure 3

An increase in serum threonine is required for muscular lipid accumulation in rats fed a low-Lys diet. Six-week-old male Wistar rats were fed ad libitum for 2 weeks with experimental diets, as indicated in A–C, D, and E, or with single amino acid–deficient diets as indicated in Figure 1. A, B, and E, lipids were extracted from the skeletal muscles with methanol-chloroform solution according to Folch's method, and the TAG content was measured. The results are shown as μg TAG per 100 mg wet weight of MLT. n = 3, means ± S.E., ∗p < 0.05 versus CN. C, serum amino acid concentrations were measured using LC MS/MS and are shown in a radar chart as fold of CN. D, the serum amino acid profiles were classified by SOM analysis and displayed on a map. Muscular TAG levels are shown in the map as a color index. Red indicates high and blue indicates low.