Fig. 1.

Separation of NBD-C6 from NBD-TAG. A: The different amounts of NBD-C6 (n = 4) and NBD-TAG (n = 4) were incubated separately in buffer K at 37°C for 1 h and subjected to lipid extraction as described in Materials and methods. NBD fluorescence in top, aqueous phase was measured. B: Different amounts of NBD-C6 (n = 3) were mixed with a fixed amount of NBD-TAG (1,250 pmol) and incubated in buffer K at 37°C for 1 h. NBD fluorescence in aqueous phase was measured after lipid extraction. Data representative of four independent experiments.