FIG. 4.

Morphological phenotypes of the pac1 bni1 and nip100 bni1 mutants. Cells of haploid strains OHNY1 (wild type [WT]), BTY3 (bni1), PFIY1 (pac1), PFIY5 (pac1 bni1), PFIY11 (nip100), and PFIY15 (nip100 bni1) were incubated at 24°C in YPDAU for 13 h, fixed, and doubly stained with DAPI and an anti-α-tubulin monoclonal antibody for DNA and cytoplasmic microtubules (FITC), respectively. (A) Quantitation of the nuclear migration defect. The percentage of cells with nuclear migration defects was determined in large-budded and unbudded cells. (B) Cytoplasmic microtubule orientation in cells with two nuclei within the mother cell of the pac1 bni1 and nip100 bni1 mutants. (C) Nuclear migration defect in large-budded cells of the pac1 bni1 and nip100 bni1 mutants. All fields were photographed at the same magnification.