TABLE 3.
Viability of mutants carrying mutations in combination with bni1 mutationa
| Genotypeb | No. of tetrads analyzed | No. of predicted mutants | % of lethal mutants | Viability of double mutant |
|---|---|---|---|---|
| pac1 bni1 | 19 | 23 | 100 | Synthetic lethal |
| nip100 bni1 | 20 | 26 | 100 | Synthetic lethal |
| arp1 bni1 | 29 | 22 | 100 | Synthetic lethal |
| cin8 bni1 | 25 | 26 | 19 | Viable |
a
PFIY1 (pac1::URA3), PFIY11 (nip100::URA3), AFIY1 (arp1::URA3), and CFIY1 (cin8::URA3) were crossed to BTY3 (bni1::HIS3), and the resulting diploids were subjected to tetrad analysis.
b
Genotypes of the inviable segregants were inferred from those of the viable segregants.