RT lysis mix

Reagent	Final concentration	Amount
First-Strand Buffer (5×)	1×	60 μL
IGEPAL CA-630 (10%)	0.3%	9 μL
dNTPs (25 mM)	0.5 mM	6 μL
DTT (0.1 M)	3 mM	10 μL
Tris-HCl [pH 8.0] (1 M)	20 mM	15 μL
Murine RNase inhibitor	1.3 U/μL	10 μL
SuperScript III RT enzyme	20 U/μL	15 μL
nuclease-free water	n/a	25 μL
Total	n/a	150 μL