Table 2.
Experimental conditions
| Condition | Description of culture conditions | Reference |
|---|---|---|
| Standard control | Medium A+ at 38°C, illuminated at 250 μmol photons m−2s−1, sparged in air with 1% (v/v) CO2, with cells harvested at OD730nm = 0.7. | (Ludwig and Bryant, 2011) |
| Dark oxic | Incubated in darkness prior to harvest, sparged in N2 | (Ludwig and Bryant, 2011) |
| Dark anoxic | Incubated in darkness prior to harvest | (Ludwig and Bryant, 2011) |
| High light | Illuminated at 900 μmol photons m−2 s−1 prior to harvest | (Ludwig and Bryant, 2011) |
| OD 0.4 | Harvested at OD730nm = 0.4 | (Ludwig and Bryant, 2011) |
| OD 1.0 | Harvested at OD730nm = 1.0 | (Ludwig and Bryant, 2011) |
| OD 3.0 | Harvested at OD730nm = 3.0 | (Ludwig and Bryant, 2011) |
| OD 5.0 | Harvested at OD730nm = 5.0 | (Ludwig and Bryant, 2011) |
| Low O2 | Sparged in N2 | (Ludwig and Bryant, 2011) |
| Low CO2 | Sparged with air [0.035% (v/v) CO2] | (Ludwig and Bryant, 2012a) |
| N-limited | Cells washed in medium A (lacking NO3−) and resuspended | (Ludwig and Bryant, 2012a) |
| S-limited | Cells washed with MgCl2 | (Ludwig and Bryant, 2012a) |
| PO43- limited | Cells washed without (PO43-) harvested at OD 730 nm = 0.7 | (Ludwig and Bryant, 2012a) |
| Fe-limited | Cells washed in medium A with 720 μM deferoxamine me- sylate B added at OD730nm = 0.35 |
(Ludwig and Bryant, 2012a) |
| NO3- | Standard growth in medium A (lacking NaNO3) with 25 mM HEPES, 1 μM NiSO4, 12 mM NaNO3 | (Ludwig and Bryant, 2012a) |
| NH3 | Standard growth in medium A (lacking NaNO3) with 25 mM HEPES, 1 μM NiSO4 and 10 mM NH4Cl | (Ludwig and Bryant, 2012a) |
| CO(NH2)2 | Standard growth in medium A (lacking NaNO3) with 25 mM HEPES, 1 μM NiSO4 and 10 mM CO(NH2)2 | (Ludwig and Bryant, 2012a) |
| Heat Shock | 1 h heat shock at 47°C | (Ludwig and Bryant, 2012b) |
| 22°C | Standard growth at 22°C | (Ludwig and Bryant, 2012b) |
| 30°C | Standard growth at 30°C | (Ludwig and Bryant, 2012b) |
| Oxidative stress | 5 μM methyl viologen added 30 min prior to harvesting | (Ludwig and Bryant, 2012b) |
| Mixotrophic | Medium A+ supplemented with 10 mM glycerol | (Ludwig and Bryant, 2012b) |
| Low salt | Medium A+ containing 3 mM NaCl and 0.08 mM KCl | (Ludwig and Bryant, 2012b) |
| High salt | Medium A+ containing 1.5 M NaCl and 40 mM KCl | (Ludwig and Bryant, 2012b) |