DEVELOPMENTAL BIOLOGY Correction for “CHD7 and Runx1 interaction provides a braking mechanism for hematopoietic differentiation,” by Jingmei Hsu, Hsuan-Ting Huang, Chung-Tsai Lee, Avik Choudhuri, Nicola K. Wilson, Brian J. Abraham, Victoria Moignard, Iwo Kucinski, Shuqian Yu, R. Katherine Hyde, Joanna Tober, Xiongwei Cai, Yan Li, Yalin Guo, Song Yang, Michael Superdock, Eirini Trompouki, Fernando J. Calero-Nieto, Alireza Ghamari, Jing Jiang, Peng Gao, Long Gao, Vy Nguyen, Anne L. Robertson, Ellen M. Durand, Katie L. Kathrein, Iannis Aifantis, Scott A. Gerber, Wei Tong, Kai Tan, Alan B. Cantor, Yi Zhou, P. Paul Liu, Richard A. Young, Berthold Göttgens, Nancy A. Speck, and Leonard I. Zon, which was first published September 3, 2020; 10.1073/pnas.2003228117 (Proc. Natl. Acad. Sci. U.S.A. 117, 23626–23635).
The authors note that “the primer sequences listed to quantify myb expression by qPCR actually target runx1 instead. We were unable to verify that the correct myb primers were used in the experiment, but two additional independent experiments (Fig. 1C quantification of myb:GFP+ cells and Fig. S2A whole-mount in situ hybridization for myb) validate our conclusion that myb expression is increased in chd7 morphant embryos.”
The authors note that on page 23634, left column, fourth full paragraph, line 12, “qPCR primers used were the following: myb forward—5′-CCGACAGAAGCCGGATGA-3′ and myb reverse—5′- TGGCACTTCGCCTCAACTG-3′; runx1 forward—5′-CGTCTTCACAAACCCTCCTCAA-3′ and runx1 reverse—5′-GCTTTACTGCTTCATCCGGCT-3′.” should instead appear as “qPCR primers used were the following: runx1 forward-1—5’-CCGACAGAAGCCGGATGA-3′ and runx1 reverse-1—5′- TGGCACTTCGCCTCAACTG-3′; runx1 forward-2—5′-CGTCTTCACAAACCCTCCTCAA-3′ and runx1 reverse-2—5′ GCTTTACTGCTTCATCCGGCT-3′.” The online version has been corrected.
Due to the same error, Fig. S1 in the SI Appendix and its corresponding legend appeared incorrectly. The SI Appendix has been corrected online.
