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. 2021 Sep 20;118(39):e2102715118. doi: 10.1073/pnas.2102715118

Fig. 5.

Fig. 5.

Anti–IL-17A monoclonal targeting of IL-17A induced fibrotic and inflammatory effects in frozen shoulder. Frozen shoulder fibroblasts were pretreated with an anti–IL-17A monoclonal antibody or IgG control before exposure to IL-17A (100 ng/mL). (A) Fibroblast’s viability, BCL2 gene expression, and mitochondrial and cytosolic cytochrome C content. (B) Gene expression of matrix and inflammatory regulators. mRNA gene expression expressed as fold change following normalization to housekeeping gene (GAPDH) and then to untreated cells (dashed line). (C) Effect on MMP3 production. (D) Effect on IL-6, IL-8 and CCL20 production. All results are mean ± SD, n = 4 control and frozen shoulder fibroblasts, * indicates significant difference from untreated cells, *P < 0.05, **P < 0.01, ****P < 0.001, #P < 0.05, ##P < 0.01, ####P < 0.0001. All statistical analyses use ANOVA with Bonferroni or Dunn’s correction for multiple comparisons depending on normality.