Fig. 1.

Clp1 mutant mice have motor function impairment and spinal cord neuron loss. (A) Motor tests. (Left) Wire-hang average latency to fall across three trials. (Center) Treadmill walking time expressed as a percentage of time facing forward. (Right) Rotarod average latency to fall across three trials. One-way ANOVA with Tukey’s multiple comparisons tests (1-mo-old mice) and t tests with Welch’s correction (5-mo-old mice). (B) Immunofluorescence for ChAT and NeuN in C6 cervical spinal cord. High-magnification images (Right) of ChAT in boxed region. (Scale bars, 100 µm.) (C) Average number of cells per hemisection of C6 spinal cord. t tests with Welch’s correction. Mean + SD. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001.