Fig. 5.

The transcriptional dynamics in different genotypes of MAF and MAR gene expression in response to vernalization. Log₂ FPKM (fragments per kilobase of transcript per million mapped reads) for orthologs of FLC, MAF, and MAR genes before, during, and after vernalization for A. thaliana (A), A. montbretiana (B), and A. alpina Pajares (C), and A. alpina pep1-1 growth in long days with no vernalization harvested weekly from 2 to 7 wk after germination (D). For A–C, shoot apices were harvested before vernalization (2 wk for Arabidopsis and A. montbretiana or 6 wk for A. alpina Pajares), during vernalization treatment (2, 4, 6, 8, 10, and 12 wk), and 1 wk after plants were returned to warm temperatures. The blue box indicates the duration of vernalization. The genes are ordered according to their chromosomal position and not their evolutionary relationships. Asterisks indicate significant differences calculated using DESeq2 of the fold changes versus the first time point. AaMARa, which does not encode an active protein, is not represented.