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. 2021 Sep 16;118(39):e2025451118. doi: 10.1073/pnas.2025451118

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Copyright © 2021 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 2. — A platform for detection of receptor–ligand interactions in the context of endogenous membranes. (A) Schematic of the RDIMIS workflow. The library of STM proteins, expressed as Fc-tagged ectodomains, are immobilized on plates. Independently, rEVs are isolated from the conditioned media of cells expressing the receptor-of-interest alongside gag-Rluc. Receptor-rEVs are screened against the collection of plate-bound STM proteins using a semiautomated workflow. Interactions between rEVs and ectodomains in the library are detected using luminescence. (B) Results show two independent RDIMIS screens for PVR gD-GPI rEVs. (C) RDIMIS screens for full-length (FL) PVR rEVs compared with repeat 2 from B.