DSS-induced colitis is mitigated in P2rx4−/−
Entps8−/− mice. Wild-type, Entpd8−/−, P2rx4−/−, or P2rx4−/−
Entpd8−/− mice were administered 3% DSS for 7 d. (A) The DAI scores of wild-type (n = 16), Entpd8−/− (n = 11), P2xr4−/− (n = 8), and P2xr4−/−
Entpd8−/− (n = 8) mice (mean values ± SD). **P < 0.01, ****P < 0.001. ##P < 0.01. *P denotes significance between wild-type and Entpd8−/− mice. #P denotes significance between Entpd8−/− and P2xr4−/−
Entpd8−/− mice. All data are from three independent experiments. (B) Representative sections (Left) and histopathological scores (Right) of the colon from wild-type (n = 18), Entpd8−/− (n = 10), P2xr4−/− (n = 14), and P2xr4−/−
Entpd8−/− (n = 9) mice. All data are mean values ± SEM from three independent experiments. **P < 0.01, ***P < 0.005, ****P < 0.001; n.s., not significant. (Scale Bars, 100 μm.) (C) Frequencies of the indicated innate myeloid cell types in the large-intestinal lamina propria of wild-type (n = 12), Entpd8−/− (n = 10), P2xr4−/− (n = 8), and P2xr4−/−
Entpd8−/− (n = 7) mice (mean values ± SEM). All data are from two independent experiments. *P < 0.05, ***P < 0.005, ****P < 0.001; n.s., not significant.