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. 2021 Sep 21;118(39):e2100594118. doi: 10.1073/pnas.2100594118

Fig. 5.

Fig. 5.

Promotion of glycolysis in neutrophils through P2X4 receptor. (A) A heat map (Left) of the genes related with glycolysis in the Gr-1+ CD11b+ cells stimulated with or without ATP-γS for 2.5 h. Scheme of glycolytic pathway (Right). (B) Relative abundances of Glu-6-P m+6 and total Glu-6-P in colonic Ly6G+ CD11b+ neutrophils cultured in the presence of U-13C6 glucose with or without 100 μM ATP-γS for 3 h. Glu-6-P; glucose 6-phosphate. These data are pooled from five independent experiments. ****P < 0.001. (C–F) The ECAR (C and E) and OCR (D and F) in Gr-1+ CD11b+ cells from the colons of wild-type or P2rx4−/− mice following pretreatment with or without ATP-γS for 3 h. The bar graphs in E and F show the average values of basal and maximum ECAR (E) and OCR (F) from four wells. All data are representative of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001; n.s., not significant.