HeLa’s standard cell culture medium

Reagent	Final concentration	Amount
Dulbecco’s Modified Eagle Medium (DMEM)	88%	44 mL
Fetal Bovine Serum (FBS)	10%	5 mL
Penicillin-Streptomycin	1%	0.5 mL
GlutaMAX	1%	0.5 mL
Total	n/a	50 mL