HeLa’s resting medium to induce cell cycle arrest (optional)

Reagent	Final concentration	Amount
Dulbecco’s Modified Eagle Medium (DMEM)	96%	48 mL
Fetal Bovine Serum (FBS)	2%	1 mL
Penicillin-Streptomycin	1%	0.5 mL
GlutaMAX	1%	0.5 mL
Total	n/a	50 mL