Reagent | Final concentration | Amount |
---|---|---|
HEPES 152 mM pH 8.2 | n/a | 1492 μL |
CuSO4 solution (100 mM stock) | 1 mM | 20 μL |
Amino Guanidin (500 mM stock) | 50 mM | 200 μL |
Ascorbic acid (250 mM) | 25 mM | 200 μL |
AlexaFluor647-azide (10 mM stock) | 10 μM | 2 μL |
Glucose 50% | 2% | 80 μL |
Glox solution (1000X) | 0.1% | 6 μL |
Total | n/a | 2 mL |