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. 2021 Sep 30;2(4):100865. doi: 10.1016/j.xpro.2021.100865

Click Chemistry buffer solution

Reagent Final concentration Amount
HEPES 152 mM pH 8.2 n/a 1492 μL
CuSO4 solution (100 mM stock) 1 mM 20 μL
Amino Guanidin (500 mM stock) 50 mM 200 μL
Ascorbic acid (250 mM) 25 mM 200 μL
AlexaFluor647-azide (10 mM stock) 10 μM 2 μL
Glucose 50% 2% 80 μL
Glox solution (1000X) 0.1% 6 μL
Total n/a 2 mL