Table 2. Crystallization.
| Method | Vapour diffusion, sitting drop |
| Plate type | Intelli-Plates (Hampton Research) |
| Temperature (K) | 291 |
| Protein concentration (mg ml−1) | 10 |
| Buffer composition of protein solution | 20 mM Tris pH 7.8, 100 mM NaCl, 2 mM DTT, 1 mM EDTA |
| Composition of reservoir solution | Na HEPES + MOPS (acid), ethylene glycols, PEG 8000 |
| Volume and ratio of drop | 0.5 µl (1:1 protein:reservoir), 0.45 µl (2:1 protein:reservoir) |
| Volume of reservoir (µl) | 50 |