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. 2021 Sep 21;77(Pt 10):334–340. doi: 10.1107/S2053230X21009195

Table 2. Crystallization.

Method Vapour diffusion, sitting drop
Plate type Intelli-Plates (Hampton Research)
Temperature (K) 291
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 20 mM Tris pH 7.8, 100 mM NaCl, 2 mM DTT, 1 mM EDTA
Composition of reservoir solution Na HEPES + MOPS (acid), ethylene glycols, PEG 8000
Volume and ratio of drop 0.5 µl (1:1 protein:reservoir), 0.45 µl (2:1 protein:reservoir)
Volume of reservoir (µl) 50