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. 2021 Sep 21;77(Pt 10):328–333. doi: 10.1107/S2053230X21008943

Table 2. Crystallization.

Method Vapor diffusion, sitting drop
Plate type MRC 2 well UVP (SwissSci)
Temperature (K) 293
Protein concentration (mg ml−1) 15
Buffer composition of protein solution 20 mM HEPES pH 7.5, 200 mM NaCl
Composition of reservoir solution 100 mM Tris–HCl pH 8.5, 200 mM MgCl2, 20%(w/v) PEG 8000
Volume and ratio of drop 1 µl:1 µl
Volume of reservoir (µl) 200