Table 2. Crystallization.
Method | Vapor diffusion, sitting drop |
Plate type | MRC 2 well UVP (SwissSci) |
Temperature (K) | 293 |
Protein concentration (mg ml−1) | 15 |
Buffer composition of protein solution | 20 mM HEPES pH 7.5, 200 mM NaCl |
Composition of reservoir solution | 100 mM Tris–HCl pH 8.5, 200 mM MgCl2, 20%(w/v) PEG 8000 |
Volume and ratio of drop | 1 µl:1 µl |
Volume of reservoir (µl) | 200 |