Table 3.
Primary and secondary antibodies list.
| Antibody | Source | Catalog number | Dilution |
|---|---|---|---|
| Rabbit anti-CDX2 | Cell Marque | EPR2764Y | 1:100 |
| Mouse anti-CDX2 | BioGenex | MU392-UC | 1:300 |
| Rabbit anti-SATB2 | Cell Marque | EP281 | 1:100 |
| Goat anti-E-Cadherin | R&D systems | AF648 | 1:400 |
| Rabbit anti-MUC2 (replaced sc 15334 from Santa Cruz) | Cloud Clone | PAA705Hu01 | 1:100 |
| Alexa Fluor 647 Donkey anti-Mouse | Thermo | A31571 | 1:500 |
| Alexa Fluor 546 Donkey anti-Mouse | Thermo | A10036 | 1:500 |
| Alexa Fluor 488 Donkey anti-Rabbit | Thermo | A21206 | 1:500 |
| Alexa Fluor 488 Donkey anti-Goat | Thermo | A11055 | 1:500 |