Figure 4.

(A) Novel object recognition test: memory retention was tested 24 h after training. Data are mean ± SEM exploratory preferences during training (left columns) or test (white and gray columns; F, familiar; N, novel) trials (n = 8 per group). (B) Immunoreactive Aβ burden in the cerebral cortex (CCx) and hippocampus (Hipp) of PEG-[G1]-CO₂H versus PEG-[G1]-DMHCA treated mice. (C) Positive immunoreactive neurons were quantified using the Image Pro Plus software. (D) Hippocampal homogenates were resolved by Western blots using the 6E10 antibody; a particular reduction of the 12 kDa band, referred as Aβ trimers (p ≤ 0.05,*, independent t test) was observed (E). Lipid plasma profile shows not statistically significant differences (independent t test) in the levels of cholesterol (F) and triglycerides (G) among groups. Statics analysis were performed using Graph-Pad Prism 6. All probability values were two-tailed; a level of 5% was considered significant. Data are reported as the mean ± SEM.