Figure 2.

The rescue of C&E defects in gpc4 mutants by endodermal expression of GFP-Gpc4 is dependent on the endoderm. (A–H) Bright-field images of the indicated embryos. Lateral view. Lines with double arrows indicate posterior body length, and dashed lines represent points used to measure the posterior body length. (I) Posterior body length in embryos shown in A–D. (J) Posterior body length in embryos shown in E–H. Data from all embryos (gray circles) and separate experiments (different blue shapes) are superimposed, with the number of embryos indicated. Each blue shape represents the mean of data from one experiment. Statistical analyses were performed using the mean of the data from individual experiments. Data are mean ± SEM. #, P > 0.05; **, P < 0.01; ****, P < 0.0001; unpaired Student's t test.