Figure 4.

The rescue of mesodermal C&E by endodermal expression of GFP-Gpc4 is mediated in part by Wnt5b and Wnt11f2. (A and B) Coimmunoprecipitation experiments were performed using lysates from HEK cells transfected with Myc or Myc-Mmp14b (negative controls), or Wnt5b-Myc or Wnt11f2-Myc and Flag-Gpc4 or Flag-JNK (negative controls) using anti–C-MYC antibody coupled to protein G magnetic beads. Western blotting of in both pellets (IP) and cell lysates (Lysate) was performed using anti-Flag and anti–C-MYC antibodies. Mw, molecular weight. (C–J) Bright-field images of the indicated control embryos and embryos injected with a subdose of MOs targeting wnt11f2/5b (5 and 1 ng). Lateral view. Lines with double arrows indicate posterior body length. Lines of the same color are equal in length. (K) Average posterior body length in embryos shown in C–J, from two independent experiments (represented by different color dots), with the number of embryos indicated. P values in different colors correspond to the embryos with posterior body length marked by the line of that color. Data are mean ± SEM. #, P > 0.05; **, P < 0.01; ****, P < 0.0001; unpaired Student's t test.