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. 2021 Sep 30;220(12):e202009082. doi: 10.1083/jcb.202009082

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© 2021 Hu et al.

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Figure S3. — GPI cleavage of Gpc4 does not drive mesoderm rescue. (A–B′) Bright-field images of groups of embryos obtained from incrossing gpc4^+/⁻ fish, uninjected (controls; A), injected with Flag-gpc4 RNA (B). (A′ and B′) Magnified images from the rectangular areas outlined by red dashed lines in A and B. Red asterisks, gpc4^−/− embryos exhibited shorter body axis (A and B), which was significantly rescued in those gpc4^−/− embryos injected with Flag-gpc4 RNA (A′ and B′). (C–F) Bright-field images of the indicated embryos at 27 hpf. (G–H′) Expression of foxa3, as detected by WISH, in the indicated embryos at 48 hpf, showing the morphology of the gut, liver (L), and pancreas (P). (G and H) Dorsal view. (G′ and H′) Lateral view.