Video 3.
GFP-Gpc4–labeled protrusions emanated from endodermal cells transport Wnt11f2-mCherry. Confocal time-lapse experiments were performed on Tg(sox17:GFP-gpc4) embryos wnt11f2-mCherry RNA at 3 somite stage using a Zeiss LSM880 confocal microscope with a LD C-Apo 40×/NA 1.1 water objective (shown in Fig. 7 B). Z-stacks of 14.45 µm were acquired at 0.85-µm intervals every 30 s using the following settings: Fast Airyscan mode, zoom 1.3, 1,024 × 1,024 pixels, 4 averaging. The movies were generated from five z-planes and are played at two frames/s. Yellow arrowheads, Wnt11f2-mCherry–expressing puncta (in magenta) on the extending protrusions.