Video 5.
Mem-mCherry labeling protrusions from Wnt-mNeonGreen-expressing cells deliver Wn5b-mNeonGreen or Wn11f2-mNeonGreen to BFP-expressing receiving cells. Confocal time-lapse experiments were performed on WT embryos mosaically injected with various RNAs at 50% epiboly, using a Zeiss LSM880 confocal microscope with a LD C-Apo 40×/NA 1.1 water objective (shown in Fig. S4, C and D). Z-stacks of 22–41.25 µm were acquired at 1–1.65-µm intervals every 60 s using the following settings: Fast Airyscan mode, zoom 1.0–1.1, 1,064 × 1,064 to 1172 × 1172 pixels, 2 averaging. The movies were generated from two or three z-planes and are played at two frames/s. White arrowheads, Wnt-mNeonGreen–expressing puncta on mem-mCherry–labeled protrusions.