Video 6.
Protrusions from Wnt-mNeonGreen–expressing donor endodermal cells deliver Wn5b-mNeonGreen or Wn11f2-mNeonGreen to neighboring host endodermal cells. Confocal time-lapse experiments were performed on Tg(sox17:mem-mCherry) embryos with Wnt-expressing donor endodermal cells at 2 somite stage using a Zeiss LSM880 confocal microscope with a LD C-Apo 40×/NA 1.1 water objective (shown in Fig. 8, A–C). Z-stacks of 12.6–22.8 µm were acquired at 0.9–1.2-µm Z intervals every 25–35 s using the following settings: Fast Airyscan mode, zoom 1.2, 1,012 × 1,012 pixels, 4 averaging. The movies were generated from three or four z-planes and are played at two frames/s. White arrowheads, Wnt-mNeonGreen–expressing puncta on mem-mCherry–labeled protrusions extending from endodermal cells.