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GFP-Gpc4-labeled protrusions in in gpc4−/− embryos are suppressed by Lat B. Confocal time-lapse experiments were performed on gpc4−/−/Tg(sox17:GFP-gpc4) embryos at 4 somite stage using a Zeiss LSM880 confocal microscope with a LD C-Apo 40×/NA 1.1 water objective (shown in Fig. S6, J and K). Images were acquired at 30-s intervals using the following settings: zoom 1.0, 1,024 × 1,024 pixels, 9 speed, 4 averaging. The movie is played at five frames/s.
