Figure 3.

Pupillometry and VR in head-fixed mice. A, Setup for head-fixed pupillometry in VR in the awake mouse, showing the habituation phase (left), in which only the virtual corridor is shown, and the stimulation phase, in which a visual stimulus appears above the virtual corridor, in the binocular portion of the visual field. B, The average fluctuations of pupillometry and velocity in all experimental mice. Dashed gray areas represent the onset and duration of auditory stimuli. C, Average event-related transients for both pupil size and velocity. Colored areas represent stimulus onset and duration. Red areas in the top part of the plot represent statistically significant data points. D, Sensibility of the system to detect locomotor-induced arousal fluctuations. Average pupil size is significantly affected by the behavioral states of the animal. During running epochs (Moving), the pupil is significantly more dilated than during the resting state (Stationary). ***p > 0.001.