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. 2021 Sep 30;8(5):ENEURO.0122-21.2021. doi: 10.1523/ENEURO.0122-21.2021

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Copyright © 2021 Mazziotti et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 6. — Offline movies analysis. A, Awake head-fixed mouse running on a treadmill, recorded for 40 s. The gray area represents a blink, and the trace of the blink is removed and linearly interpolated (red line). B, Awake mouse during two-photon calcium imaging. Here a brighter pupil is clearly visible with respect to A. Blinking epochs are removed and linearly interpolated. C, Pupillometry performed on a human subject, with a higher blinking rate with respect to mice. In all figures, the inset images represent the ROIs.