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. 2021 Oct 4;21(5):11. doi: 10.1093/jisesa/ieab072

Table 2.

Stability ranking of the seven reference genes using four different algorithms

ΔCq geNorm NormFinder BestKeeper RefFinder
Gene SD Gene M Gene SV Gene r Gene GM
β-actin 0.95 β-actin 0.29 GAPDH 0.31 GAPDH 0.972 β-actin 3.15
RPL40 1.02 RPL40 0.31 β-actin 0.33 β-actin 0.961 GAPDH 3.46
GAPDH 1.03 GAPDH 0.32 RPL40 0.45 EF1-β 0.953 RPL40 3.59
ARF1 1.13 18SrRNA 0.39 18SrRNA 0.49 RPL40 0.942 β-TUB 4.03
18SrRNA 1.15 β-TUB 0.48 EF1-β 0.56 β-TUB 0.932 EF1-β 5.12
EF1-β 1.23 EF1-β 0.55 β-TUB 0.79 ARF1 0.904 18SrRNA 5.69
β-TUB 1.31 ARF1 0.69 ARF1 0.94 18SrRNA 0.877 ARF1 6.37

GM, geometric mean; M, average of expression stability values; r, Pearson correlation coefficient; SV, stability value.