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. 2021 Oct 4;22:282. doi: 10.1186/s13059-021-02493-x

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — Functional validation of alx4a and gbx2 in iridophore development. a Lateral view of WT and alx1KO, alx3 KO, alx4b KO fish. b Lateral view of alx4a CRISPR-mediated knockout fish. c Iridophore detection in 4 dpf larvae of WT and alx4a knockout larvae. White arrows mark iridophores in WT larvae. d Lateral views of 1 dpf larvae, 2 dpf larvae, and adult fish comparing WT to Tg(miniCoopR-alx4a) and Tg(miniCoopR-gbx2) fish. e Representative pictures and quantification of iridophores from 3 dpf larvae tail trunks of WT (n = 21), Tg(miniCoopR-alx4a) (n = 20), and Tg(miniCoopR-gbx2) (n = 20). P values were calculated with two-tailed Welch’s t-test. Error bars represent ± SE. f Lateral whole-body view of iridophore rescue in three mosaic tg(miniCoopR-alx4a;alx4aKO) fish. Black box denotes the zoomed region in picture below