Fig. 1.

Identification of keratinocytes and fibroblasts. A The purity of primary keratinocytes and fibroblasts was identified by flow cytometry. B The relative gene expression levels of known marker genes of keratinocytes and fibroblasts were detected by qRT-PCR. The relative mRNA expression was expressed as 2^−ΔΔCT, the Gapdh gene served as an internal reference to normalize the target gene expressions. Data are presented as the mean ± SD from at least three independent experiments. *P < 0.05, **P < 0.01