TABLE 1.
Pairwise interactions between wild-type and mutant forms of Scd2 and Shk1 tested in the yeast two-hybrid assay
| GAD fusion protein | β-Galactosidase activity with indicated GBD fusion proteina
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Scd2 | Scd2ΔN92 | Scd2Δ 114-177 | Scd2ΔN184 | Scd2 [1-188] | Scd2 [1-113] | Scd2 [113-188] | Cdc42 | Byr2 | |
| Shk1 | + (20.26) | + (30.55) | − (0.11) | − (0.11) | + (57.26) | − (0.10) | + (89.22) | + | − |
| Shk1ΔN118 | − (0.29) | − | − | − | + | − | + (12.32) | + | − |
| Shk1R1 | + (27.20) | + | − | − | + | − | + (44.70) | − | ND |
| Shk1R2 | − (0.22) | − | − | − | − | − | − (0.10) | + | ND |
| Shk1R3 | − (0.18) | − | − | − | + | − | + (3.05) | − | ND |
| Scd1[40-872] | + (109.00) | + | + | + | − | − | − (0.11) | ND | − |
| Ras1 | − (0.17) | − (0.15) | − (0.15) | − (0.10) | − (0.14) | − (0.09) | − (0.12) | − | + |
a
+ and − indicate whether β-galactosidase activity was detected between pairs of GBD and GAD fusion proteins in the β-galactosidase filter assay (see Materials and Methods). At least eight independent transformants were tested for each determination made by the filter assay. Values in parentheses indicate β-galactosidase activity detected by the quantitative liquid β-galactosidase assay (see Materials and Methods). Values are expressed as Miller units and represent the average activity assayed for two independent yeast transformants. ND, not determined.