FIGURE 3.
Effect of pharmacological inhibitors on ubiquitin conjugates in cortical neurons from wild type mice. Quantitation and representative immunoblots of ubiquitin conjugates (PolyUb) in neurons after 24 hr treatment with 10 μM or 50 μM of (a, b) 3E,5E-bis[(4-nitrophenyl) methylene]-1-(1-oxo-2-propen-1-yl)-4-piperidinone, (c, d) IU1, or (e, f) IU1-47 showing dose-response effect on levels of poly-ubiquitin conjugates. Quantitations are shown relative to levels observed in neurons treated with DMSO alone (0 μM). β-tubulin was used as a loading control. n = 3 independent cortical neuron extracts. Data are shown as mean ± SEM. Significance is reported relative to treatment with DMSO, with the exception of IU1-47, which is shown relative to 10 μM inhibitor. **p < .01